Reaction of chloroacetyl-CoA with rabbit fatty acid synthase
نویسندگان
چکیده
منابع مشابه
Acetoacetyl-CoA reductase activity of lactating bovine mammary fatty acid synthase.
Fatty acid synthase, purified from lactating bovine mammary gland, utilizes coenzyme A esters of acetoacetic, 3-hydroxybutyric, and crotonic acids as substrates for its partial reactions at micromolar concentrations. The NADPH:acetoacetyl-CoA reductase had a Km of 5 microM acetoacetyl-CoA and a Vmax of about 4 mumol of NADPH oxidized min-1 mg-1. In contrast, the Km for the model compound, aceto...
متن کاملStudies on acetyl-CoA carboxylase and fatty acid synthase from rat mammary gland and mammary tumours.
The activities of two lipogenic enzymes, acetyl-CoA carboxylase and fatty acid synthase, were determined in two transplantable mammary adenocarcinomas (13762 and R3230AC) carried by non-pregnant, pregnant and lactating rats, and in mammary tissue of control animals (non-tumour-carrying) of comparable physiological states. During mammary-gland differentiation of control or tumour-carrying animal...
متن کاملMolecular Pathways: Fatty Acid Synthase.
Therapies that target tumor metabolism represent a new horizon in anticancer therapies. In particular, cancer cells are dependent on the generation of lipids, which are essential for cell membrane synthesis, modification of proteins, and localization of many oncogenic signal transduction enzymes. Because fatty acids are the building blocks of these important lipids, fatty acid synthase (FASN) e...
متن کاملTransacylase activity of lactating bovine mammary fatty acid synthase.
An assay for the transacylation reaction catalyzed by fatty acid synthase was developed which does not require model substrates or labelled acyl-derivatives of CoA. It involves the transfer of the acyl group from unlabelled CoA to [3H]CoA. This assay shows the occurrence of transacylation at a relatively high rate with a variety of substrates that the enzyme is able to utilize. The activity is ...
متن کاملFIRE (fatty acid synthase insulin-responsive element) and ICE (inverted CCAAT element) regulate fatty acid synthase.
Introduction Fatty acid synthase (FAS) plays a central role in de novo lipogenesis in mammals and birds by catalysing the reaction in the conversion of acetyl-CoA and malonyl-CoA into palmitate. FAS activity is not known to be regulated by allosteric effectors or covalent modifications. However, FAS concentration is exquisitely sensitive to nutritional, hormonal and developmental states [ 1-31....
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1982
ISSN: 0014-5793
DOI: 10.1016/0014-5793(82)81054-5